thr 77 - An Overview
thr 77 - An Overview
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Antibodies sure to the blocking peptide now not bind to the epitope around the focus on protein. By evaluating the staining through the blocked antibody versus the antibody by yourself, one can see which staining is distinct.
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This guidebook outlines further achievable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
This tutorial outlines further achievable optimization of mobile and immunoassay parameters to ensure the best possible effects are...
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind particularly towards the target antibody and block antibody binding. These peptide generally consists of the epitope recognized by the antibody.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S phase.
Overexpression of the gene is noticed in lots of tumors, which results in chromosome instability, and therefore might add to tumorigenesis. This protein was identified to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in promoting cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been explained. Two additional splice variants have been claimed but in depth nucleotide sequence information and facts isn't nevertheless available.
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